| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Townsend, Rachel A (17)
|
MRBGNW |
24.55 |
26.23 |
1.68 |
| 1 |
Gonzalez, Angelina (17)
|
MRMPNY |
25.64 |
26.23 |
0.59 |
| 3 |
Zhu, Catherine (16)
|
MRBGNW |
27.20 |
27.23 |
0.03 |
| 4 |
Kleinsmith, Margaret T (16)
|
MRBGNW |
25.71 |
27.70 |
1.99 |
| 5 |
McHugh, Amanda K (17)
|
MRBGNW |
25.92 |
28.71 |
2.79 |
| 6 |
Forrest, Jasmine (15)
|
MRSWAG |
27.50 |
29.13 |
1.63 |
| 7 |
Ford, Sydney E (16)
|
MRBGNW |
28.29 |
29.17 |
0.88 |
| 8 |
Negron, Lorielle (15)
|
MRSWAG |
29.34 |
29.86 |
0.52 |
| 9 |
Laurenceau, Alyssa M (15)
|
MRYMID |
27.87 |
29.89 |
2.02 |
| 10 |
Chia, Molly (15)
|
MRYMID |
29.39 |
30.01 |
0.62 |
| 11 |
Gnad, Julia M (15)
|
MRHS |
29.28 |
30.18 |
0.90 |
| 12 |
Kamal, Sarah M (15)
|
MRBGNW |
28.69 |
30.99 |
2.30 |
| 13 |
Canales, Carolyn M (17)
|
MRHS |
29.99 |
31.16 |
1.17 |
| 14 |
Economakos, Stavroula (15)
|
MRHS |
29.00 |
31.42 |
2.42 |
| 15 |
Karam, Stephanie (15)
|
MRSWAG |
31.72 |
32.52 |
0.80 |
| 16 |
Koestler, Aimee J (17)
|
MRBGNW |
31.80 |
33.26 |
1.46 |
|
Nieves, Talitha L (17)
|
MRSWAG |
27.49 |
NS |
- |